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Biotechnology
Year: 2010  |  Volume: 9  |  Issue: 3  |  Page No.: 368 - 372

Enhanced Development of Embryogenic Callus in Stevia rebaudiana Bert. by Additive and Amino acids

A. Das and N. Mandal    

Abstract: Stevia rebaudiana Bert. is an economically important medicinal plants act as a sugar substitute for diabetic and obese people. In the present investigation a novel protocol was developed to accelerate the production of embryogenic calli so that large number of in vitro plant regeneration can be feasible. Two different types of amino acids (Trypthphan and Glutamine) in different concentration and three different types of additives (yeast extract, casein hydrolysate and Potato extract) were utilized along with MS medium supplemented with 2,4-D (2.0 mg L-1), BAP (0.5 mg L-1), sucrose (30 g L-1) and agar with leaf as explant to elucidate their role in developing and maintaining embryogenic calli. It was found that glutamine (50 mg L-1) and casein hydrolysate (100 mg L-1) produced greenish, healthy nodular calli with more embryogenic potential and less necrotic lesion in comparison with only PGR supplemented basal medium which further performed better in regeneration medium for producing large number of regenerated shoots.

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