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Biotechnology
Year: 2010  |  Volume: 9  |  Issue: 2  |  Page No.: 141 - 148

Induction of Morphogenetic Callus and Multiple Shoot Regeneration in Ceropegia pusilla Wight and Arn.

R. Kondamudi, V. Vijayalakshmi and K. Sri Rama Murthy    

Abstract: This study was undertaken to evaluate the most suitable concentration of plant growth regulators and perfect explant (node, internode and thin cell layer explants-TCLs) for callus induction and subsequent organogenesis in an endangered medicinal Ceropegia pusilla. The best callus induction was found on the MS medium supplemented with 6-benzylaminopurine (BAP) 13.32 μM L-1 + 2, 4-dichlorophenoxy acetic acid (2,4-D) 0.45 μM L-1 from TCLs. After the initiation of the callus, it was immediately transferred to MS medium supplemented with BAP along with other auxins like 2, 4-D, Indol-3-Acetic Acid (IAA), Indole-3-Butyric Acid (IBA), Naphthalene Acetic Acid (NAA). The regenerative calli were raised on the MS medium supplemented with 1.13 μM L-1 of 2,4-D. Whereas, the organogenic calli was raised on the medium containing 22.7, 40.86, 45.4 μM L-1 of Thidiazuron (TDZ) induced 37.54±0.29, 37.12±0.18 and 34.32±0.17 shoots, respectively. On the media containing BAP 13.32 + IBA 0.49 to 1.23 μM L-1 micro shoots rooted best and 75% of the shoots were survived. The plantlets were established, acclimatized and thrived in green house conditions with 80%. The regeneration protocol developed in this study provides a basis for germplasm conservation and for further investigation of bio active constituents of this medicinal plant.

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