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Year: 2009  |  Volume: 8  |  Issue: 4  |  Page No.: 425 - 433

Optimization of Fermentation Conditions for the Biosynthesis of Inulinase by the New Source; Aspergillus tamarii and Hydrolysis of Some Inulin Containing Agro-Wastes

W.I.A. Saber and Noura E. El-Naggar    

Abstract: From the rotted Jerusalem artichoke tubers, 11 fungi were isolated on synthetic medium containing inulin as a sole carbon source. On the base of inulinase activity on inulin (I), one of them was selected and identified as Aspergillus tamarii AR-IN9. Incubation of A. tamarii AR-IN9 for 72 h, pretreatment of inulin-containing agro-wastes in autoclave at 20 Ib/in2, 3% corn steep liquor in the growth medium, pH 5.5 and 35°C were the best conditions for inulinase production. The overall production reached up to 71.97 U mL-1. Aspergillus tamarii AR-IN9 showed invertase activity on sucrose (S), with high values of I/S ratio which indicating that the fungus is active in inulinase production. Inulinase activity reached its maximum at pH 5.2 and 45°C. The enzyme was still stable by 80% or more at the pH range from 4.4 to 7.2 for 24 h and by 75% at 50°C for 90 min. The metal ions; MgCl2, CoCl2 and MnCl2 positively modulated inulinase activity. The resultant inulinase showed high hydrolysis activity on Jerusalem artichoke (71.64%), dahlia tubers (67.55%) and chicory roots (55.11%). Therefore, various agro-wastes and inulin-containing materials could be economically hydrolyzed with A. tamarii AR-IN9 inulinase into fructose, which has many therapeutic and industrial aspects. Besides the beneficial environmental impact by the bioremediation of such agro-wastes.

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