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Biotechnology
Year: 2009  |  Volume: 8  |  Issue: 2  |  Page No.: 281 - 284

Evaluation of TaGSK1 Gene Expression in Selected Wheat Genotypes as Salinity Marker Assisted Selection

S. Bahrami, M. Solouki, B. Siasar and A. Ghanbari    

Abstract: In order to determine the level of TaGSK1 gene expression in 9 selected wheat genotypes an experiment was carried out in Agricultural Biotechnology Institute, University of Zabol. Seeds of wheat cultured on solid MS medium plate under positive and control condition and RNA was extracted for each genotype and treatment. Relative gene expression using cDNA as template was done in Real Time PCR. The results of real time PCR showed that the Bam genotype has maximum level expression of TaGSK1 gene between 9 genotypes. Minimum expression was found in ER-Salt-85-17 line originated from Tehran Province. TaGSK1 gene expression between genotypes is varied from 39% in ER-Salt-85-17 until 71% in Bam genotypes.

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