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Biotechnology

Year: 2009  |  Volume: 8  |  Issue: 2  |  Page No.: 212 - 219

Improved Production of Endoglucanase Enzyme by Aspergillus terreus; Application of Plackett Burman Design for Optimization of Process Parameters

Gahda A. Youssef and Mahmoud M. Berekaa

Abstract

In this study, bagasse was used as substrate for endoglucanase (carboxymethyl-cellulase; CMCase) production using locally isolated Aspergillus terreus and the culture parameters were optimized for enhancing cellulase yield. The fungus showed 0.9 U mL-1 endoglucanase (CMCase) activity, during growth on basal salts medium at 35oC, initial pH value of 5.5 and in presence of 5% bagasse as a sole c-source. Preliminary experiments to address the most suitable nitrogen source as well as the optimal substrate (bagasse) treatment revealed that the optimal enzyme activity were 2.1 and 2.43 U mL-1 in presence of 3 g yeast extract and 1 N HCl or 1 NaOH, respectively. Statistically based experimental design was applied to optimize the production of endogluconase by A. terreus. To evaluate the effect of different culture conditions on the production of CMCase enzyme, Plackett-Burman factorial design was carried out. Twelve variables were examined for their significance on enzyme production. Treated bagasse (T2), non treated bagasse (NT), K2HPO4, NaNO3, trace elements, KCl, temperature and pH were the most significant factors encourage CMCase enzyme production, whereas treated bagasse (T3), yeast extract and MgSO4, were the most significant factors decreasing CMCase enzyme production. The pre-optimized medium showed approximately 4 folds increase in cellulase enzyme production.

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