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Year: 2008  |  Volume: 7  |  Issue: 4  |  Page No.: 781 - 786

Genetic Diversity of Tunisian Barley Accessions Based on Microsatellite Markers

F. Guasmi, L. Touil, K. Feres, W. Elfelah, T. Triki and A. Ferchichi    

Abstract: Genetic diversity can be measured by several criteria, including phenotype, pedigree, allelic diversity at marker loci and allelic diversity at loci controlling phenotypes of interest. Abundance, high level of polymorphism and ease of genotyping make simple sequence repeats (SSRs) an excellent molecular marker system for genetics diversity analyses. In this study, we used a three of mapped SSRs to examine the genetic diversity of Tunisian barley accessions and to establish phylogenetic relationships among them. These primers produce a total of 9 bands witch 7 loci polymorphic, the percentage of polymorphism ranged from 14.28 to 42.85%. The clustering grouped the studied accessions into 3 clusters with no correlation to geographical origins. The CFA permit to group the various populations by projected them in a plan formed by two axes (F1, F2), present 3 groups which are similar with those obtained by hierarchical classification. Present results demonstrate that this SSR marker was highly informative and was useful in generating a meaningful classification of barley germplasm.

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