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Biotechnology
Year: 2008  |  Volume: 7  |  Issue: 4  |  Page No.: 763 - 768

Molecular Typing of Iranian Cladosporium Isolates Using RAPD-PCR

Siamak Haddadi, Ali R. Khosravi, Mina Rostamibashman, Ali Masoudi-Nejad, Saeed Soltani and Minoo Soltani    

Abstract: Fourteen isolates of Cladosporium were collected from different areas and clinical samples. These isolates were genotypically compared using RAPD (Random Amplified Polymorphic DNA) technique. The data showed high degree of polymorphism between clinical and non clinical isolates and quite low polymorphism within isolates of the same group (clinical or non clinical group). The RAPD`s results were subjected to a numerical taxonomy analysis, using UPGMA method. A phenogram was constructed for isolates Based on its structure; we concluded that genotypic data provide enough information to use the unweighted pair-group method to cluster the isolates in accordance to their respective group. The phenogram grouped in a single branch the isolates of C, G, H, K, E, J, I, M, N and F, indicating a great similarity between these fungi that are both clinical and non clinical ones and suggesting that the classification as distinct isolates may not be appropriate for these isolates of the Cladosporium.

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