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Biotechnology
Year: 2008  |  Volume: 7  |  Issue: 4  |  Page No.: 710 - 717

Production and Biochemical Characterization of an Extracellular Lipase from Rhizopus chinensis CCTCC M201021

Natascha Pogori, Ahmad Cheikhyoussef, Yan Xu and Dong Wang    

Abstract: The aim of the study was to investigate the extracellular lipase production by the fungus, Rhizopus chinensis CCTCC M201021. The biochemical characteristics of this lipase were also identified. The inducing effect of vegetable oils (soybean oil, sunflower oil, sesame oil, rice bran oil and olive oil), fatty acids (palmitic acid, oleic acid, linoleic acid and stearic acid) and surfactants (Tween 80, sodium dodecyl sulphate, gum arabic and Triton X-100) on lipase production by Rhizopus chinensis CCTCC M201021 was investigated. Soybean oil enhanced lipase production by 102% and was the highest among other oils studied. There was no significant effect on lipase production by surfactants at p<0.05. The unsaturated fatty acids, oleic and linoleic acid significantly enhanced the lipase production by 147%. The optimum temperature for lipase activity was 30°C and the optimum pH was 6.0. The enzyme had stability in the pH range of 4.0 to 10.0 for 1 h. The enzyme retained stability of 60% of the maximum at 70 °C after pre-incubation for 1 h. Zn2+ and Cu2+ ions depressed the lipase activity but Mg2+ and Ca2+ ions were found to stimulate the lipase activity. The enzyme had broad substrate specificity towards both p-nitrophenyl esters and triglyceride substrates since it efficiently hydrolyzed long-chain and short-chain fatty acids but greater preference was observed for the long-chain fatty acids. The lipase was able to hydrolyze both the 1, 3-position and the internal position of triolein.

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