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Biotechnology
Year: 2008  |  Volume: 7  |  Issue: 3  |  Page No.: 487 - 492

Plasmid DNA of Antibiotic Producing Strains of Streptomyces sannanensis Isolated from Different States in Southern India

P. Ponmurugan and B. Nithya    

Abstract: Soil samples were collected from different states in Southern India to isolate and characterize actinomycetes at molecular levels through plasmid DNA and protein pattern. A total of 12 soil samples were collected from which four strains (AP-1, KN-2, KL-3 and TN-4) were isolated and characterized as Streptomyces sannanensis. The antimicrobial activity of these strains was studied against gram-negative and positive-bacteria. It was capable of producing antibiotic against gram-positive, while gram-negative bacteria were not affected. The potential of antibiotic production of these strains is likely to be chromosomally encoded by confirming the detection of plasmid DNA. The strains such as KN-2 and KL-3 showed two plasmids and the other two strains showed only one. This is a preliminary step to correlate the chemotaxonomic relationship among the strains of Streptomyces spp. for secondary metabolite production.

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