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Biotechnology
Year: 2008  |  Volume: 7  |  Issue: 2  |  Page No.: 258 - 265

Transformation of Maize with Trehalose Synthase Gene Cloned from Saccharomyces cerevisiae

Dan Tao, Yu Mu, Feng-Ling Fu and Wan-Chen Li    

Abstract: A new sequence of trehalose synthase gene TPS1 was cloned from strain AS.1416 of Saccharomyces cerevisiae by the method of homologous amplification. Sequence analysis showed that its similarity with formerly reported sequence of gene TPS1 (X68496) was as high as 99.3%. The putative protein of this sequence had the same conserved contigs with the protein sequences of trehalose synthases in many eukaryotic and prokaryotic organisms. This sequence was used as exotic gene to construct a stress-inducible expression vector and transform embryonic calli of maize mediated by agrobacterium. After screening and regeneration, one fertile plant was detected to be positive by specific PCR amplification and sequencing of the amplified product.

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