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Biotechnology
Year: 2007  |  Volume: 6  |  Issue: 1  |  Page No.: 53 - 56

Use of Polymerase Chain Reaction for Detecting Banana Bunchy Top Nanovirus

A.M. Galal    

Abstract: Banana Bunchy Top Nanovirus (BBTV), the causal agent of Banana Bunchy Top Disease (BBTD) is the most serious viruses infecting banana allover the world as well as in Egypt. Polymerase Chain Reaction (PCR) as a molecular tool was used for detection of BBTV in banana samples showed the characteristic symptoms of BBTV as well as in symptomless banana samples. Two oligonucleotides belonging to the BBTV-component 2 resulted the full length were used as primers at 50°C annealing temperature. DNA was extracted from the tested samples and three DNA concentrations, i.e., 50, 100 and 150 ng were used as templates. A PCR fragment with a size of 1056 bp was amplified in case of virus-infected banana samples. As an interesting result, the tested samples were varied in their PCR results according to the DNA concentrations and this could be reflect the low concentration of the viral DNA with the total DNA extracted from the virus-infected samples. Furthermore, the presence of the BBTV-DNA was also detected in the total DNA extracted from the viruliferous banana aphid (Pentalonia nigronervosa Coq). The results also confirmed the use of PCR for BBTV detection in particularly the coast was not limited.

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