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Biotechnology
Year: 2007  |  Volume: 6  |  Issue: 4  |  Page No.: 465 - 472

In vitro Root Formation in Micropropagated Shoots of Jojoba (Simmondsia chinensis)

Muhammad Azhar Bashir, Muhammad Akbar Anjum and Hamid Rashid    

Abstract: The study was conducted to initiate roots from in vitro derived shoots of six promising strains of jojoba i.e., PKJ-1 to PKJ-6. MS medium was supplemented with three auxins i.e., IBA, IAA and NAA each at the rate of 1.25, 2.5 and 5.0 mg L-1 in the one experiment and at the rate of 0.5, 1.0 and 1.5 mg L-1 in the other experiment. Different growth parameters pertaining to root formation were recorded. In the 1st experiment, the lowest concentration (1.25 mg L-1) of each auxin gave satisfactory results, the other two higher concentrations of auxins (2.5 and 5.0 mg L-1) caused callus induction. Efficacy of auxins was improved in the 2nd experiment as each auxin and its concentrations affected significantly the root characteristics of each strain. IBA and 0.5 mg L-1 were the most effective auxin and concentration, respectively. Among the strains, PKJ-3 performed the best of all, as it took the minimum time to root, produced maximum number of roots and attained longer primary root and higher percentage of rooted shoots in both experiments.

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