Asian Science Citation Index is committed to provide an authoritative, trusted and significant information by the coverage of the most important and influential journals to meet the needs of the global scientific community.  
ASCI Database
308-Lasani Town,
Sargodha Road,
Faisalabad, Pakistan
Fax: +92-41-8815544
Contact Via Web
Suggest a Journal
Biotechnology
Year: 2007  |  Volume: 6  |  Issue: 3  |  Page No.: 420 - 425

Optimization of in vitro Conditions for Callus Induction, Proliferation and Regeneration in Wheat (Triticum aestivum L.) Cultivars

Sikandar, Waqar Ali, Israr Khan and Iqbal Munir    

Abstract: Tissue culture conditions for two varieties of wheat (Triticum aestivum L.), Fakhr-e-Sarhad and Gandam-2002 were optimized using mature seeds as source of explant. Both of these varieties were tested for their callus production, callus proliferation and regeneration abilities. Different concentrations of 2,4-D (2,4-Dichlorophenoxy acetic acid) were tested for callus induction. Both of these varieties showed maximum callus induction (46.87% for Fakhr-e-Sarhad and 62.5% for gandam-2002) on MS medium supplemented with 3.5 mg L-1 2,4-D. Excellent callus proliferation (87.5% for Fakhr-e-Sarhad and 95.31% for Gandam-2002) was obtained on MS medium containing 4 mg L-1 2,4-D, also callus proliferation (81.25% for Fakhr-e-Sarhad and 93.75% for Gandam-2002) was found on 3 mg L-1 BAP (Benzyl aminopurine). Both varieties were regenerated into whole plant on medium containing 1 mg L-1 IAA (Indol, 3-acetic acid) in combination with 2 mg L-1 BAP after a culture period of 3 weeks. The regeneration was 50 and 60% for Fakhr-e-Sarhad and Gandam-2002, respectively on medium containing 3 mg L-1 Kinetin. The Gandam-2002 produced an average of 4 plantlets per callus, while the Fakhr-e-Sarhad produced an average of 2 plantlets per callus on medium fortified with 1 mg L-1 IAA along with 2 mg L-1 BAP.

Cited References   |    Fulltext    |   Related Articles   |   Back
   
 
 
 
  Related Articles

 
 
 
 
 
 
 
 
 
Copyright   |   Desclaimer   |    Privacy Policy   |   Browsers   |   Accessibility