Asian Science Citation Index is committed to provide an authoritative, trusted and significant information by the coverage of the most important and influential journals to meet the needs of the global scientific community.  
ASCI Database
308-Lasani Town,
Sargodha Road,
Faisalabad, Pakistan
Fax: +92-41-8815544
Contact Via Web
Suggest a Journal
Biotechnology
Year: 2007  |  Volume: 6  |  Issue: 3  |  Page No.: 339 - 343

Lateral Bud Culture of Papaya (Carica papaya) for Clonal Propagation

M.M. Rohman, Md. Nazrul Islam, Md. Shameul Alam, Munshi Rashid Ahmad and Tapan Kumar Paul    

Abstract: In vitro culture of lateral buds of field grown mature plants were tested in MS medium supplemented with different concentrations of BAP and NAA. Seasonal endophytic contamination was suppressed by shaking propagules for 2 h in 300 mg L-1 rifampicin before surface sterilization. Maximum survival rate was found in MS medium supplement with 1.0 mg L-1 BAP plus 0.20 mg L-1 NAA. The highest number of shoots was produced in MS medium containing 0.50 mg L-1 BAP and 0.20 mg L-1 NAA. Both the highest multiplication rate and the longest shoot were found in MS medium supplemented with 0.50 mg L-1 BAP plus 0.20 mg L-1 NAA. On the same treatment an increasing trend was observed in multiplication rate of shoot upto 5th subculture which decreased thereafter. First subculture produced the highest shoot length which decreased with the increase in number of subculture. Half strength MS medium supplemented with 1.0 mg L-1 IBA was found as the best treatment to produce root in the culture media. Rooted plantlets were transplanted successfully.

Cited References   |    Fulltext    |   Related Articles   |   Back
 
 
   
 
 
 
  Related Articles

 
 
 
 
 
 
 
 
 
Copyright   |   Desclaimer   |    Privacy Policy   |   Browsers   |   Accessibility