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Year: 2007  |  Volume: 6  |  Issue: 3  |  Page No.: 309 - 315

In vitro Shoot Multiplication of Six Promising Strains of Jojoba (Simmondsia chinensis)

Muhammad Azhar Bashir, Hamid Rashid and Muhammad Akbar Anjum    

Abstract: Nodal segments, 1.5-3.0 cm long with 1 or 2 nodes taken from in vitro shoot cultures of six jojoba strains, were cultured on MS medium supplemented with 2.5 or 5.0 mg L-1 BA or 2.5 mg L-1 BA + 2.5 mg L-1 of one of NAA, IAA or IBA. Different growth parameters regarding shoot multiplication were recorded. BA at the rate of 2.5 mg L-1 caused the earliest sprouting of buds and produced the longest primary shoot, maximum number of nodes per primary shoot. However, BA at the rate of 5.0 mg L-1 produced the maximum number of shoots per explant. While the combination consisting of 2.5 mg L-1 BA + 2.5 mg L-1 IBA delayed the bud sprouting and produced the minimum number of shoots, the shortest primary shoot and the minimum number of nodes per primary shoot. Among the strains PKJ-3 led the other strains in performance, while PKJ-2 trailed in all parameters. The number of shoots and the length of primary shoot were significantly affected by the interactions between strains and growth regulators combinations.

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