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Year: 2007  |  Volume: 6  |  Issue: 2  |  Page No.: 165 - 174

In vitro Shoot Initiation from Nodal Explants of Jojoba (Simmondsia chinensis) Strains

Muhammad Azhar Bashir, Hamid Rashid and Muhammad Akbar Anjum    

Abstract: Nodal segments of six promising strains of jojoba i.e., PKJ-1 to PKJ-6 were cultured in vitro on solidified MS medium supplemented with BA and Kinetin, alone or in combinations at different concentrations to initiate shoot growth. Different growth parameters regarding shoot initiation and growth were recorded during the experimental period of three months. The study revealed that BA (alone) was better than Kinetin (alone) or BA + Kinetin combinations. The lowest concentration of BA (1.25 mg L-1) proved more effective for many shoot parameters and PKJ-3 strain was the most responsive to cytokinins alone or in combination. Some explants also developed callus at the highest level (5.0 mg L-1) of BA (alone) or in combinations of BA with Kinetin. Many explants were vitrified at the highest level (5.0 mg L-1) of Kinetin (alone).

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