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Biotechnology
Year: 2006  |  Volume: 5  |  Issue: 3  |  Page No.: 353 - 357

Reliable DNA Extraction on Historical Malaria Negative Smears Conducted to Nested PCR in South-East of Iran

A. Ebrahimzadeh, M. Polshakan, M.I. Qureshi and I. Sharifi    

Abstract: The reliability of PCR depends on many factors, such as high quality DNA pellets obtained from fixed Giemsa-stained blood samples, good reagents and adequate conditions of amplification. In account of this limitations, a retrospective epidemiological study for malaria diagnosis was conducted in a hypoendemic area, Sistan and Baluchestan province of south-east of Iran. For the first time this research designed to perform a nested PCR protocol using new and modified method of DNA extraction from negative Fixed and Giemsa Stained smears. This study demonstrated 11.55% PCR positive for Plasmodium vivax and Plasmodium falciparum which were undetectable by microscopy.

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