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Biotechnology

Year: 2004  |  Volume: 3  |  Issue: 1  |  Page No.: 56 - 62

A Comparative Study of Axillary Shoot Proliferation from the Nodal Explants of Three Varieties Pummelo (Citrus grandis [L.] Osb.)

F. Begum, M.N. Amin, S. Islam and M.A.K. Azad

Abstract

Nodal segments of in vitro germinated seedlings of three pummelo varieties [Var.-1 (pulp is pink colour), Var.-2 (pulp is white colour) and Var.-3 (pulp is red colour)] were cultured on half-strength MS medium for axillary shoot proliferation. A large number of shoot buds were produced when such four weeks old culture were subcultured on half-strength MS medium containing 1.0 mg l-1 BAP. Roots were induced when the isolated individual shoots were cultured on half-strength MS medium containing 0.1 mg l-1 each of NAA, IBA or IAA. Cent percent root were observed on half-strength MS medium having 0.1 mg l-1 NAA. These in vitro grown plantlets were then successfully transferred to outside natural condition through successive phases of acclimatization. About 93% of the regenerated plantlets survived under ex vitro condition.

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