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Biotechnology
Year: 2004  |  Volume: 3  |  Issue: 2  |  Page No.: 194 - 199

Effect of Culture Medium on Shoot Initiation from Calluses of Different Origin in Potato (Solanum tuberosum L.)

Muhammad Akbar Anjum and Hakoomat Ali    

Abstract: Attempts were made to regenerate shoots from calluses of different origin in potato on different media. Shoot initiation from growing tuber calluses on the medium of Iapichino et al.[1] was about 45 days earlier than on the medium of Ahloowalia[2] in both the potato cultivars i.e. Maris Piper and Desiree. Frequency of calluses producing shoots and number of shoots produced per callus were higher on the medium of Iapichino et al.[1] as compared to that of Ahloowalia[2] and in Cv. Maris Piper than the Cv. Desiree. Attempts to regenerate shoots on the medium of Jarret et al.[3] proved unsuccessful. Shoot regeneration from leaf calluses of both Desiree and Maris Piper was achieved on all the three media tested. Shoots were initiated earlier from the calluses subcultured on the medium of Iapichino et al.[1] followed by that of Lam[4], while it took the longest time on the medium of Ahloowalia[2]. The frequency of calluses producing shoots and number of shoots produced per callus were higher on the medium of Iapichino et al.[1] and the lowest on the medium of Ahloowalia[2]. The performance of Cv. Maris Piper was better because regeneration frequency was higher and also more number of shoots per callus was regenerated in this cultivar. Shoot regeneration from calluses derived from suspension-cultured cells of stem origin was 10-15 days earlier from the calluses subcultured on the medium of Bokelmann and Roes[5] than from those subcultured on the medium of Lam[4] in both Desiree and Maris Piper. Frequency of calluses producing shoots was higher in Cv. Maris Piper. The number of shoots regenerated per callus was also higher on the medium of Bokelmann and Roest[5] than on the medium of Lam[4], probably because these media contained different growth regulators in different concentrations.

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