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Biotechnology
Year: 2004  |  Volume: 3  |  Issue: 2  |  Page No.: 187 - 193

Effect of Culture Medium on Direct Organogenesis from Different Explants of Various Potato Genotypes

Muhammad Akbar Anjum and Hakoomat Ali    

Abstract: Stem segments (about 1 cm in length and without axillary buds) of four potato genotypes, i.e. two cultivars of Solanum tuberosum, Desiree and Maris Piper and of two wild species, S. commersonii and S. acaule and tubers explants of the two cultivars of S. tuberosum were cultured on three different regeneration media, as developed by Jarret et al.[1], Iapichino et al.[2] and Ahloowalia[3]. The medium of Iapichino et al.[2] took less time to initiate shoots from stem explants, while shoot regeneration from tuber explants was earlier on the medium of Ahloowalia[3]. Among the genotypes, shoot regeneration (when occurred) was in general quicker in Maris Piper. Shoots from the stem explants of S. commersonii were only regenerated on the two media i.e., of Iapichino et al.[2] and Ahloowalia[3], while the explants of S. acaule failed even to survive on either of the media tested. Regeneration frequency (%) and number of shoots regenerated in both, stem and tuber explants, were higher on the medium of Iapichino et al.[2] and in S. tuberosum Cv. Maris Piper.

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