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Biotechnology

Year: 2003  |  Volume: 2  |  Issue: 3  |  Page No.: 206 - 213

Plant Regeneration from the Cotyledonds of Tossa Jute (Corchorus olitorius L.)

Asma Khatun, Chandan Kumar Saha, Zabun Naher, Shirin Mahbub, Abu Bakar Siddique and Shahana Bilkis

Abstract

Plant regeneration from the cotyledons of C. olitorius has recently been optimized at Bangladesh Jute Research Institute (BJRI). One of the major constraints of getting plant regeneration from the explants of C. olitorius was the production of healthy seedlings in vitro. An efficient seed germination system and healthy seedling production have been established at BJRI. Seeds of different varieties (O4, O9897, OM1 and O72) of C. olitorius germinated on both agar supported hormone free MS medium and cotton supported hormone free liquid MS medium. The percentage of seeds germinated on cotton supported medium was found to be much higher than seeds germinated on agar supported medium. This system ensured maximum of an average of 98% seed germination. Whereas, in case of agar-supported medium an average of 46% seeds could be germinated with very slow growth. The seedlings grown on cotton supported medium were found to be much more healthily than seedlings grown on agar supported medium. Plant regeneration was obtained from the cotyledonary petioles of the varieties (O4, O9897, OM1 and O72) of C. olitorius on MS agar solidified medium supplemented by IAA 0.5 and BAP 3.0 mg l-1. The efficiency of plant regeneration from the cotton supported seedlings was found to be as good as the agar supported seedlings. The plantlets produced roots on hormone free MS medium readily. After transfer to soil the plants grew into maturity and produced fruits. No morphological changes were noticed.

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