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Biotechnology
Year: 2003  |  Volume: 2  |  Issue: 1  |  Page No.: 1 - 7

Optimization of Various Parameters for the Production of Glucose Oxidase from Rice Polishing Using Aspergillus niger

Hafiz M. Hamid, Khalil-ur-Rehman, M. Anjum Zia and M. Asgher    

Abstract: The enzyme glucose oxidase was produced by fermentation technology, using Aspergillus niger as fermentation organism and rice polishing as substrate. Maximum production of enzyme (3.42 U mL-1) was recovered at substrate level of 2% after 36 h of submerged fermentation. The pH for the optimal production of enzyme was found to be 4. Addition of salts such as urea (0.3%), CaCO3 (0.04%) and KH2PO4 (0.6%) into the fermentation medium enhanced enzyme production while MGSO4.7H2O was found to inhabit microbial growth and glucose oxidase production by A. niger.

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