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Biotechnology
Year: 2002  |  Volume: 1  |  Issue: 1  |  Page No.: 34 - 39

Production of Amyloglucosidase by UV Irradiated Strain of Aspergillus niger

Ikram-ul-Haq, Shahida Javed and Hamad Ashraf    

Abstract: The parental strain of Aspergillus niger was subjected to UV irradiations for 5-30 minutes. One hundred and ten mutants of Aspergillus niger were isolated by observing the hydrolysis of starch in the petri plates. Of all the mutants tested, the mutant strain of Aspergillus niger GCBU-25, isolated after 25 minutes of UV irradiation gave the maximum production of AMG (136.1 IU/ml/min), which gave two fold increase in the production of the enzyme than the parental strain. The production of amyloglucosidase reached its maximum (183 IU/ml/min) when starch at 1% level and (NH4)2SO4 at 0.2% level of nitrogen was used as carbon and nitrogen source respectively.

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