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Acta Agriculturae Scandinavica, Section A - Animal Sciences

Year: 2009  |  Volume: 59  |  Issue: 4  |  Page No.: 204 - 210

Expression, characterization, and variation of the porcine calpain 7 gene

X. -Q. Yang, L. -J. Guo, C. -Y. Zhai, H. Yu, H. Liu and D. Liu

Abstract

This study was designed to clone the porcine calpain 7 gene, CAPN7, to characterize its tissue expression, and to investigate its genetic variation. The cDNA sequence that contains a 2442 bp open reading frame encoding 813 amino acids was obtained by reverse transcription-polymerase chain reaction and rapid amplification of cDNA ends methods based on in slico cloning and homologous cloning, and five introns were identified. Quantitative real-time PCR showed that CAPN7 was ubiquitously expressed in all tissues examined, and the mRNA level in muscles was low at 1-7-days-old and high from 90 to 270-days-old. In addition, two single nucleotide polymorphisms in coding sequence were identified and the polymerase chain reaction-restriction fragment length polymorphism methods were established to detect the polymorphisms. At each site, the distribution of three genotypes is significantly different (P<0.01) in Yorkshire, Duroc, Wild boar, and Min pigs.

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