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Asian Journal of Scientific Research
Year: 2019  |  Volume: 12  |  Issue: 2  |  Page No.: 202 - 210

Phytochemical Screening and Antibacterial Activity of Eucalyptus camaldulensis’s Leaves and Bark Extracts

Azzah Ibrahim Alghamdi and Ibtisam Mohammed Ababutain    

Abstract: Background and Objective: Choosing the appropriate antibiotics (bacteriostatic or bactericidal) considered the most important aspect of treatment. This study aimed to determine the type of inhibitory activity of Eucalyptus camaldulensis’s leaves and bark extracts whether it is bacteriostatic or bactericidal. Materials and Methods: The antibacterial activity of E. camaldulensis leaves and bark extracts were assessed against four bacterial isolates (Pseudomonas aeruginosa ATCC27853, Escherichia coli ATCC25922, Bacillus subtilis and Staphylococcus aureus ATCC24213). Minimum Inhibitory Concentration (MIC) was estimated using a 2-fold dilution method and Minimum Bactericidal Concentration (MBC) was estimated using a pour plate method. The bactericidal effect was estimated by calculating the ratio between MIC and MBC. Qualitative phytochemical assays were performed using a standard method and GC-MS analysis. Results: The results showed that the E. camaldulensis extracts have inhibitory activity against all tested bacteria. The results also indicated that the MBC/MIC ratios of E. camaldulensis extracts were one to two-fold dilutions which are considered as a bactericidal effect. The result of qualitative phytochemical assays showed the presence of tannin, saponins, flavonoid, carbohydrate and protein compounds in all leaves and bark extracts. The results of the GC-MS analysis showed that E. camaldulensis extracts possess a variation in chemical constituents. Some of these compounds have an anti-microbial effect like Cyclononasiloxane, octadecamethyl-, 17-Pentatriacontene, 4H-Pyran-4-one, 3,5-dihydroxy-2-methyl- and Phytol. Conclusion: E. camaldulensis may be used as a natural antibiotic for its bactericidal effect to treat serious infections caused by pathogenic bacteria.

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