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Asian Journal of Scientific Research
Year: 2019  |  Volume: 12  |  Issue: 1  |  Page No.: 120 - 125

Curcuma Explant Multiplication in MS Medium Supplemented with Various Kinds of Cytokinine and Auxin Concentration

Erni Suminar, Mita Indriani, Sulistyaningsih, Denny Sobardini, Noladhi Wicaksana and Syariful Mubarok    

Abstract: Background and Objectives: Establishing new protocol for in vitro propagation of turmeric from vegetative tissue is needed to be studied more due to the demand of turmeric increased every year and also for producing a faster rhizome propagation and free-disease rhizome. The MS solid medium mostly used in in vitro culture. To make a faster explant multiplication, cytokinine must be supplemented to MS medium. The objective of this study was to study the effect of various cytokinins and concentration of Naphthalene Acetic Acid on the explant multiplication of turmeric. Materials and Methods: This study was conducted in the Laboratory of Tissue Culture, Faculty of Agriculture, Universitas Padjajaran. Young shoot of sprout rhizome was cultured in MS basal medium supplemented with different kinds of cytokinins (BAP, TDZ and Zeatin) were combined with 0.01 and 1.0 mg L–1 of NAA. All parameters were tested by analysis of t-student test at the 5% significance level. Results: Three kinds of cytokinins (BAP, TDZ and Zeatin) were combined with 0.01 and 1.0 mg L–1 of NAA and supplemented to MS solid medium. The result showed that TDZ was more effective than BAP and Zeatin for explant multiplication. The supplementation of TDZ combined with NAA resulted the best effect on shoot multiplication resulted in the highest shoot number compared with control but the supplementation of cytokinins affected in the root elongation resulted the short root length. Conclusion: It was concluded that supplementation of cytokinine in MS medium significantly effects on the shoot formation but inhibit the root elongation. The TDZ was the best type of cytokinine that affects shoot multiplication.

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