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Asian Journal of Scientific Research

Year: 2008  |  Volume: 1  |  Issue: 5  |  Page No.: 525 - 531

Monoclonal Antibody Production: Media Optimization for Enhancement the Cell Viability of Hybridoma Cell

Maizirwan Mel, Maizura Mat Saad, Yumi Zuhanis Hasyun Hashim and Mohamad Ramlan Mohamed Salleh


Media optimization of RC1 hybridoma cell culture for monoclonal antibody production was carried out in T-Flask experiment. The three identified important variables to affect the cell viability were studied. By using Central Composite Design of Response Surface Methodology (STATISTICA v 6.1) has shown that cell viability was mainly affected by glutamine, serum and NaCO3 concentration, respectively. Among the 16 runs tested, Run 11 indicated the best viability of the cell (>80% for five days). The critical values were obtained at 13.5, 1.68 and 0.87% for serum, glutamine and NaCO3, respectively. These data were very significant where the p-values obtained for glutamine, serum and NaCO3 were 0.000069, 0.003968 and 0.342151 (R2 = 0.95476 and R Adj. = 0.88691), respectively.

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