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Asian Journal of Scientific Research
Year: 2008  |  Volume: 1  |  Issue: 2  |  Page No.: 122 - 129

Genotoxic Potential of Agricultural Soils of Amritsar

J.K. Katnoria, S. Arora and A. Nagpal    

Abstract: The present study was planned to estimate the content of some heavy metals and evaluate the genotoxic potential of extracts of soil samples collected from different agricultural fields of Amritsar, India, employing Ames test and Allium cepa root anaphase aberration assay (Al-RAAA). The water extraction of soil samples was carried out using distilled water (soil: water, 1:2 w/v). The extracts were evaporated to dryness and redissolved in distilled water to prepare different concentrations corresponding to 0.25, 0.5, 1.0, 2.0 and 2.5 g equivalent of soil per plate and were used with Salmonella typhimurium TA98 and TA100 strains, with and without in vitro metabolic activation (S9) to detect direct and indirect mutagenic effects. For Al-RAAA, different concentrations of extracts (10, 25, 50, 75 and 100%) were used for treatment of root tips of A. cepa. In situ conditions were also simulated by allowing the onion bulbs to root directly in soil samples contained in small pots. The genotoxic potential of soil samples was correlated with content of heavy metals like Chromium, cobalt, copper, manganese, mercury, nickel and zinc. The pH, alkalinity, water holding capacity, bulk density, moisture content, nitrates, phosphates and potassium were also studied. It was observed that soil samples, which showed the highest percentage of aberrations, also contained higher concentrations of one or more metals studied.

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