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Asian Journal of Poultry Science
Year: 2017  |  Volume: 11  |  Issue: 1  |  Page No.: 14 - 19

SK Kedu Semen Cryopreservation in Beltsville Poultry Semen Extender and Lactated Ringer’s-Egg Yolk Extender Using Dimethyl Sulfoxide

Sipora P. Telnoni, R. Iis Arifiantini, Tuty L. Yusuf and Sri Darwati    

Abstract: Objective: This study was conducted to evaluate frozen semen quality of SK kedu chicken after cryopreservation in Beltsville Poultry Semen Extender (BPSE) and lactated ringer's-egg yolk (LR-EY) with 8, 10 and 12% concentrations of dimethyl sulfoxide (DMSO). Methodology: Semen was collected from 5 SK kedu roosters using cloaca massage technique twice a week. Semen was evaluated macro and microscopycally then polled and divided into 6 tubes of treatments. Three tubes were diluted in BPSED8, BPSED10, BPSED12 and three other tubes were diluted in LR-EYD8, LR-EYD10, LR-EYD12. Semen of each treatments loaded into 0.25 mL straw, equilibrated at 5°C for 2 h. Freeze above nitrogen vapor and stored in container of liquid nitrogen at -196°C, then semen thawed in a water bath at 37°C for 30 sec. Data was analyzed using Statistical Analysis System (SAS). Results: Result showed that post thawing sperm motility in LR-EYD10 (40.83±1.67%) was higher (p<0.05) than other treatments. The sperm recovery rate in LR-EYD10 (46.71±1.97%) was higher than other treatments (p<0.05). Sperm viability was not differ post dilution, post equilibration and post thawing in all treatments. Conclusion: This study showed that LR-EY with 10% DMSO (LR-EYD10) was better to maintain sperm motility and sperm recovery rate of SK kedu frozen semen.

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