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Asian Journal of Plant Sciences
Year: 2018  |  Volume: 17  |  Issue: 2  |  Page No.: 75 - 84

Trypsin Inhibitor Activity and Protein Analysis of Gall Rust from Sengon Plants (Falcataria moluccana Miq.) Infected with Uromycladium tepperianum Fungus

Alfi Rumidatul, Endah Sulistyawati and I Nyoman Pugeg Aryantha    

Abstract: Background and Objectives: Sengon Falcataria moluccana (F. moluccana) has the ability to synthesize protease inhibitors, such as trypsin inhibitors, that can inhibit the activity of an enzyme produced by Uromycladium tepperianum (U. tepperianum), a fungus that causes gall rust disease. The purpose of this research is to study the activity of the trypsin inhibitor, the total protein content and the protein profile in healthy and gall rust infected sengon. Materials and Methods: The samples used in this research were taken from sengon twigs consisting of healthy and gall rust infected bark and wood. In this research, three age classes of sengon were used: 1-2 years, 3-4 years and 5-6 years. The method used in this research was field sampling, trypsin inhibitor activity analysis, protein determination and protein profile analysis by SDS-PAGE (Sodium dodecyl sulfate-polyacrylamide gel electrophoresis). Results: The results showed that, in general the activity of trypsin inhibitors in the gall rust infected sengon twigs was higher than in the healthy sengon twigs. The highest protein content was found in gall rust infected bark twigs and the lowest protein content was in healthy wood twigs. The results of the SDS-PAGE analysis of healthy and gall rust infected bark showed that there was a protein with a molecular weight of 21 kDa, which was identified as a trypsin inhibitor. Conclusion: Falcataria moluccana infected with U. tepperianum fungus showed an increase in the activity of trypsin inhibitors and protein content.

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