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Asian Journal of Plant Sciences
Year: 2013  |  Volume: 12  |  Issue: 1  |  Page No.: 34 - 39

Molecular Characterization of Waxy Gene in Aegilops tauschii

Wei Li, An-Jun Liu, Yu-Zhen Sheng, Guo-Yue Cheng, Zi-En Pu and Ya-Xi Liu    

Abstract: In order to exploiting new genetic resources for the wheat breeding, nine waxy genes (granule-bound starch synthase, GBSSI) were obtained from wild species, Aegilops tauschii (DD) by special primer PCR cloning. These sequences showed the higher similarity to the sequences of Wx-D1 locus in common wheat. The analysis of variation and alignment of sequences showed that all the sequences could be distinguished, although from the same species. Out of 35 variable sites of nucleotide in the whole sequences, twelve variable sites located on the region of exon and made six amino acid residues change. Most of variable sites located on transit peptide. Using neighbour-joining method, phylogenetic tree suggested that the sequences of waxy gene from Ae. tauschii were clustered closely with the sequences of Wx-D1 locus and far with the sequence of Wx-A1 and Waxy-B1 loci in Triticum L.. Moreover, Ae. tauschii showed more close relationship with Triticum L. and barley and had more close relationship with barley and far distance with rice, potato, pea and Arabidopsis. These results would contribute to the deep understanding of functional aspects and evolution of waxy gene and the improving of starch quality in common wheat.

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