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Asian Journal of Plant Sciences
Year: 2013  |  Volume: 12  |  Issue: 1  |  Page No.: 21 - 27

Effects of 2,4-D and Kinetin on Callus Induction of Barringtonia racemosa Leaf and Endosperm Explants in Different Types of Basal Media

Z. Dhiya Dalila, Hafsah Jaafar and A. Abdul Manaf    

Abstract: Barringtonia racemosa, was a medicinal plants that widely used in traditional practice to treat various ailments and even its young leaf were eaten raw. Due to its healing properties, the tree was intensively harvested without replacement planting effort and in vitro technique can be one of the ways to propagate them in the field. The purpose of this study was thus to develop a standardized basic protocol to induce a high frequency of callus induction. The effects of 2,4-Di-chlorophenoxy-acetic acid (2,4-D) and 6-furfuryl aminopurine (kinetin) on callus induction percentage (CI%) of B. racemosa leaf and endosperm explants in Murashige and Skoog medium, Lloyd and McCown medium, Murashige and Skoog (Modified) medium were evaluated. The following three basal media were added: 30 g L-1 of sucrose, 2.5 g L-1 phytagel and combination of (2,4-D) with kinetin in various concentrations (0.5,1.0,1.5 and 2.0 mg L-1). The highest CI% was shown by B. racemosa endosperms culture, with 100% callusing in MS medium added with 1.5 mg L-1 2,4-D and 0.5 mg L-1 kinetin. The callus was friable, beige and grew intensively. In leaf culture, the highest CI% was 93.33% with friable, beige colour callus in MS medium supplemented with 2.0 mg L-1 of 2,4-D. Results indicated that 2,4-D and kinetin with certain combinations in MS medium gave better callusing compared to other basal media. These results provided a basis for the optimization of callus culture protocol to develop a reproducible of plantlet, inducing callus for somatic embryogenesis and secondary metabolite production.

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