Abstract: The present study was to investigate antioxidant, analgesic and antiulcer effect of ethanolic seeds extract of Trichosanthes cucumerina. Extraction was done by cold maceration method for 72 h using ethanol as solvent. The preliminary phytochemical screening showed the presence of triterpenoids, flavanoids and sterols. The antioxidant activity was determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging method. The extract showed maximum scavenging activity 86.2% at 150 μg mL^-1 by DPPH method as compared to standard antioxidant ascorbic acid. Further extract was studied for antiulcer activity by ethanol induced ulcer model and Non steroidal anti-inflammatory drugs induced method and analgesic activity by Eddy’s hot plate method. Ethanolic extract of Trichosanthes cucumerina cause 72.1 and 57.1% inhibition of ulcer in Non steroidal anti-inflammatory drugs induced model and ethanol induced model at 500 mg kg^-1. Result concluded that Ethanolic extract of Trichosanthes cucumerina possess high antiulcer activity which is due to high antioxidant activity and extract also showed potential analgesic activity by Eddy’s hot plate method.