Abstract: Casuarina equisetifolia is the wide spread exotic trees in peninsular Indian and well known member of the family Casuarinacea. The present study was carried out biochemical indicators of ten good rooting and poor rooting clones of Casuarina equisetifolia. The DNS and anthrone method of reducing sugar and carbohydrate was estimated respectively. The result of the analysis revealed significant difference between the good rooting and poor rooting clones with higher the content reducing sugar (6.99-12.68) in better rooting, lesser the content of carbohydrates (20.09-58.59) present in better rooting. The present investigation reveals reducing sugar and carbohydrates plays a major role in rooting of Casuarina equisetifolia.