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Asian Journal of Plant Sciences
Year: 2010  |  Volume: 9  |  Issue: 6  |  Page No.: 351 - 357

Evaluation of Cotyledonary Node of Clitoria ternatea L. for High Frequency in vitro Axillary Shoot Proliferation

J. Singh and K.N. Tiwari    

Abstract: The objective of this study was to establish mass multiplication protocol for Clitoria ternatea. An efficient protocol for in vitro propagation of the valuable medicinal and forage plant aparajita (Clitoria ternatea L.) was developed through axillary shoot proliferation. Cotyledonary node having cotyledons or devoid of cotyledons were evaluated for axillary shoot development. The effect of cytokinins, sucrose concentrations and light-dark condition on axillary shoot regeneration was investigated. Cotyledonary node with cotyledons cultured on MS medium supplemented with 1.5 mg L-1 BAP yielded the maximum number (10.8) of shoots per explant. All media supplemented with 3% sucrose and incubated under 16 h light condition found most suitable for high frequency axillary shoot proliferation. Elongated shoots produced profuse rooting after transfer to half-strength MS medium supplemented with 0.2 mg L-1 IBA. The rooted plantlets were successfully acclimatized and exhibited 95% survival after transfer into the field. This protocol is suitable for large scale production of plantlets of C. ternatea.

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