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Asian Journal of Plant Sciences
Year: 2009  |  Volume: 8  |  Issue: 3  |  Page No.: 224 - 229

Molecular Detection of a Drought Stress-Inducible D-Amino Acid Oxidase Gene from Zea mays L.

A. Gholizadeh, M.H. Faizi and B.B. Kohnehrouz    

Abstract: In this research, the induction of maize DAAO gene was investigated under drought stress conditions. RT-PCR end product analysis of the mRNA samples purified from stressed and non-stressed leaves showed that DAAO gene is only expressed in stressed plants. The results indicated that DAAO transcript is consistently detected overtime as stress conditions continued, but it fell below the limit of detection when plants are completely dried/died. This result may be consistent with the earlier reports that points the accumulation of D-amino acids and expression of DAAO activity during mammalians aging or tissue developments in which PCD is involved. Cloning and sequencing of the expressed product revealed that the induced cDNA nucleotide and deduced amino acid sequences are 100% identical to DAAO gene that is expressed when maize plants utilize D-alanine as nitrogen source. This finding may provide new insights into the active role of D-amino acid oxidase gene and lead to the ways for the new studies on plant DAAO in the future.

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