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Asian Journal of Plant Sciences
Year: 2007  |  Volume: 6  |  Issue: 5  |  Page No.: 874 - 877

Evaluation of Genetic Diversity by Using Link Maker for Amylose Content of Some Iranian Local Rice Cultivars

M.R. Rahemi, S.K. Kazemitabar, A. Moumeni and A.A. Ebadi    

Abstract: Molecular markers are the best method for evaluation of genetic diversity in life. In this study 72 cultivars those indicated types Indica and Japonica, performed at Iranian Rice Research Institute, Rasht Iran. For assessment of genetic diversity, the Waxy gene locus link to controller trait amylose content, used from two oligo nucleotide (484 and 485). Consequently, perform PCR reactions and scoring. Rice cultivars have been screened representing current important Iran germplasm using primers flanking the Waxy microsatellite. According to earned information in this study, seven classes of Waxy microsatellite, containing (CT)n repeats were detected, ranging from n = 7 to 20. The amplified PCR products ranged from 102 to 128 bp in length and represented the (CT)n repeats of (CT)7, (CT)8, (CT)14, (CT)17, (CT)18, (CT)19 and (CT)20, that according to Iranian germplasm cultivars earn seven classes for in gene locus, that regularity explained per classes of 70, 72, 78.95, 80 and 70%.

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