Effects of the Sucrose Concentration and pH Level on Shoot Regeneration from Callus in Araria elata Seem
The optimum sucrose concentration and pH level were estimated for in vitro shoot regeneration from callus of Aralia elata Seem in a Broad-leaved tree (BT) medium with different concentrations of sucrose (0.0-25.0 g L-1) and a wide range of pH levels (4.5-6.8). The highest rate of shoot production was achieved in a shoot-regenerating medium with 15 g L-1 sucrose and a pH of 5.8. Shoot multiplication and growth were significantly affected by sucrose. Shoots were not formed in a sucrose-free medium. When the pH was adjusted below or above 5.8, the growth rate of the shoot significantly decreased.
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