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Asian Journal of Plant Sciences
Year: 2007  |  Volume: 6  |  Issue: 4  |  Page No.: 617 - 622

Responses of Five Locally Adapted Sweet Potato (Ipomoea batatas (L.) Lam)Cultivars to in vitro Plant Regeneration via Direct and Indirect Embryogenesis

J.N. Oggema, J.P. Ouma and M.G. Kinyua    

Abstract: The development of a reliable plant regeneration protocol in sweet potato forms the basis for sweet potato (I. batatas) genetic improvement. The success in production of the transgenic sweet potato is dependent on the reliability and efficiency of the regeneration protocol to produce somatic embryos capable of forming whole plants. The effect of direct and indirect embryogenesis on in vitro plant regeneration was studied and thereafter a suitable tissue culture protocol for 5 locally adapted Kenyan sweet potato cultivars Mugande, SPK004, Kemb10, Japon tresmesino and Zapallo established. Embryogenic calli was induced directly and indirectly from sweet potato leaf explants and auxiliary buds cultured on MS medium supplemented with 2,4-D (0, 0.5 and 1.0 mg L-1). Absisic acid was added to induce embryo maturation and when the hormone levels were reduced these embryos began to differentiate into shoots before whole plants were regenerated. For each treatment the number and days taken to form shoots, roots and plants that were regenerated were counted and used as a selection index of an efficient sweet potato regeneration protocol for the locally adaptable Kenyan cultivars. The test cultivar had a significant (p≤0.05) effect on both direct and indirect embryogenesis. The use of indirect embryogenesis was beneficial for the local Kenyan sweet potato cultivars as more calli formed hence ensuring higher plant regeneration and increased mass propagation of in vitro plants while direct embryogenesis took a shorter time to form shoots and roots but fewer plants were regenerated.

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