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Asian Journal of Plant Sciences
Year: 2007  |  Volume: 6  |  Issue: 3  |  Page No.: 484 - 489

Optimum 2,4-D Concentration Suitable for Embryogeneic Callus Induction in Local Kenyan Sweet Potato Cultivars

J.N. Oggema, M.G. Kinyua and J.P. Ouma    

Abstract: Embryogeneic callus induction and subsequent differentiation is accomplished by application of suitable growth regulators and controlling conditions during culture. The growth regulator, 2,4-D is one of the most effective auxin for calli induction in sweet potato (Ipomoea batatas (L.) Lam.), however the optimum concentration varies with individual cultivars. The optimum 2,4-D level for the local sweet potato cultivars has not been determined yet it is important in multiplication of planting material and transformation procedures. To determine the optimum 2,4-D concentration that produced embryogenic calli early and in high frequencies for local Kenyan sweet potato cultivars Mugande, SPK004, Kemb10, Japon tresmesino and Zapallo, leaf explants were cultured in vitro on MS basal medium supplemented with six concentrations of 2,4-D (0, 0.5, 1.0, 2.0, 3.0 and 5.0 mg L-1), set as a factorial treatment arranged in a Completely Randomized Design (CRD), replicated three times. For each treatment four parameters were considered: Mean number of days taken to form calli, calli weight, calli diameter and calli strength. The results established significant (p<0.05) differences in cultivar responses to 2,4-D levels. Calli induction was effective when supplemented at low levels of 0.5 mg L-1 as high levels above 3.0 significantly reduced the quantity and quality of embryogenic calli that was formed. Low levels of 2,4-D should be used for local Kenyan sweet potato cultivars as the number of days taken to form calli reduced and calli incidences increased.

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