Abstract: Twenty-five accessions of Triticum durum originating from different geographical areas of Iran and 10 durum cultivars from European countries were evaluated for high molecular weight glutenin subunit (HMW-GS) composition using SDS-PAGE. The data indicated the prevalence of the null allele (52%) and 2* subunit (48%) at the Glu-A1 and four alleles, namely 14+15 (32%), 13+16 (28%) and 7+20 (24%) represented about 80% of the alleles at Glu-B1 locus in the Iranian durum compared to European durum. This information can be a valuable reference for designing breeding program for the improvement of breed and pasta making quality of bread and durum wheat, respectively in Iran.