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Asian Journal of Plant Sciences
Year: 2004  |  Volume: 3  |  Issue: 6  |  Page No.: 747 - 751

In vitro Regeneration and Callus Formation of Different Hybrid of the (Sunflower) Helianthus annuus L. Yielding in Turkish Trakya Region

Hayati Arda    

Abstract: Sixteen different Helianthus annuus L. hybrids growing in Turkish Trakya region were included in this study. Three different methods were employed. While germination occurred in the first method following surface sterilization which was done without removing the testa, in the second one surface sterilization was done by removing the testa. Unlike these, however, in the third one, germination occurred in moist cotton media without removing the testa. Culture experiments were done in various media with varying hormone concentrations by using different explants of the obtained samples. Productive results were obtained in cultures in which cotyledon and hypocotyl explants were used. When sections of these explants were investigated, regeneration and callus formation were found to differ between native and hybrid types.

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