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Asian Journal of Plant Sciences
Year: 2004  |  Volume: 3  |  Issue: 6  |  Page No.: 666 - 669

In vitro Micropropagation of Some Important Sugarcane Varieties of Bangladesh

M.A. Mamun, M.B.H. Sikdar, Dipak Kumar Paul, M. Mizanur Rahman and Md. Rezuanul Islam    

Abstract: In vitro micropropagation of sugarcane variety viz., Isd-28 and Isd-29 have been studied. Nutrient media containing growth regulators enhanced callus induction, shoot differentiation and root formation in vitro. For covenant experiment, surface sterilization of the explants from the field grown plants was essential and sterilization with 0.1% HgCl2 for 8 min was satisfactory. In this treatment, 85-90% of the leaf sheath explants was Contamination free. Among the explants cultured for proliferation fortified with 3.0 mg L-1 2, 4-D explants of all the 2 varieties of sugarcane showed the best performance for the callus induction after 16-20 days of culture. The concentration of BA tested 1.5 mg L-1 of the cytokinin produced comparatively higher percentage of shoot proliferation. Different concentration of BA mg L-1 in combination with different auxin viz., IAA, IBA and NAA were tested for shoot proliferation. Isd-28 and Isd-29 showed best shooting when media were supplemented with BA 1.5 mg L-1+0.5 mg L-1 NAA. In vitro proliferated shoots were rooted on MS and modified MS media. Best results of rooting ware observed on modified MS medium supplemented with auxins (NAA+IBA) 0.5 mg L-1 and 0.5 mg L-1 showed fair results. Eighty to ninety percent regenerated plantlets were viable at normal temperature with 85% humidity while transferred sterilized soil.

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