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Asian Journal of Plant Sciences

Year: 2004  |  Volume: 3  |  Issue: 4  |  Page No.: 522 - 528

Effects of Explant Type and Growth Regulators on Organogenesis in a Medicinal Tree, Phellodendron amurense Rupr.

M.A.K. Azad, S. Yokota, S. Yahara and N. Yoshizawa

Abstract

A mass in vitro propagation system for Phellodendron amurense Rupr., a medicinally important plant, has been developed. A range of a cytokinin, and auxins has been investigated for multiple shoot induction with hypocotyl, cotyledon, leaf, and internode explants. Adventitious shoot buds were induced successfully on MS basal media supplemented with various concentrations of BAP in combination of either NAA or IBA. The optimal induction of adventitious shoot buds occurred from all explants on MS media with 0.9 μM BAP, and 0.5 μM NAA or IBA. In the percentage of shoot formation, number of total shoots per culture, and usable shoots per culture, better results were obtained with hypocotyl explant, followed by cotyledon, leaf, and internode explants. Highest length of shoots was obtained with leaf explant, followed by cotyledon, hypocotyl, and internode explants. In vitro grown shoots rooted successfully on the MS media with 0.5-2.0 μM IBA, NAA, or IAA. Four subculture cycles were used, and after each subculture, percentage of shoot formation, total number of shoots, number of usable shoots, average length of shoots, percentage of root formation, total number of roots, and average length of roots per culture were recorded. Regenerants were transferred to Kanuma soil, and successfully established under ex vitro environment.

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