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Asian Journal of Plant Sciences
Year: 2004  |  Volume: 3  |  Issue: 4  |  Page No.: 484 - 488

Production and Pathogenicity of Four Steinernematids in Diamondback Moth, Plutella xylostella L. (Lepidoptera: Yponomeutidae)

A.N. Mahar, M. Munir and K.B. Laghari    

Abstract: Production and infectivity of Steinernema carpocapsae, S. abbasi, S. karii and S. riobravis (entomopathogenic nematodes) using larvae and pupae of diamondback moth were studied. Nematodes production of all species was determined by the number infective juveniles (IJs) established in DBM larvae and pupae using sand and filter paper bioassy. Maximum number of S. carpocapsae were produced at 25°C, however the production of other isolates was higher at 30°C. Infectivity tests carried out in sand media revealed that maximum IJs of S. carpocapsae were recovered from the DBM larvae and pupae at 25°C whereas other three isolates produced reasonable number of IJs at 30°C. Similarly, DBM pupae with cocoon produced higher number of IJs than without cocoon. The research indicated that application of nematodes with the knowledge of insect pest biology that represents a possible new strategy for controlling DBM larvae and pupae.

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