In vitro Response and Shoot Multiplication of Banana with BAP and NAA
Meristem culture of banana cv. BARI-I was performed for virus eradication and shoot multiplication where different concentrations of BAP (0, 1, 2, 3, 4, 5 and 6 mg L-1) and combinations of different level of BAP (0, 4 and 5 mg L-1) and NAA (0, 0.5, 1.0, 1.5 and 2.0 mg L-1) were used. Among the different levels of BAP, better response was found with 5.0 mg L-1 BAP in respect of survivability (91.67%), single shoot formation rate (62.50%) and formation of hard meristamatic ball like structure (29.17%). Maximum shoot multiplication (4.52/explant) with highest shoot length (3.62 cm) was achieved on MS medium containing 4.0 mg L-1 BAP+1.5 mg L-1 NAA treatment. The clusters of multiple shoots were also rooted on MS medium supplemented with 2.0 mg L-1 NAA.
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