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Asian Journal of Plant Sciences
Year: 2004  |  Volume: 3  |  Issue: 3  |  Page No.: 330 - 334

Somaclonal Variation in Sugarcane Through Tissue Culture and Subsequent Screening for Salt Tolerance

Shah Jehan Khan, Muhammad Ayyaz Khan, Haji Khalil Ahmad, Rahm Din Khan and Yusuf Zafar    

Abstract: Tissue culture techniques were used to develop soma-clones in sugarcane to screen salt tolerant clones subsequently. Callus masses derived from young leave of high yielding cultivar of sugarcane (CP-43/33). The plantlets were regenerated from one month old callus. The plantlets were transplanted to pots after hardening. The grown up plants were transplanted to well prepare soil. The sets of well developed plant were planted in saline sodic soil. Screening of salt tolerant soma-clones was made. The salt tolerant soma-clones performed better in the characteristics of number of tiller/plant, stem height, number of nodes/stem, root band width with variability of 20, 50, 8.33 and 14.3%, respectively. The soma-clones performed less in the characteristics of girth of stem and brix (%) of 12.5 and 5.2, respectively.

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