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Asian Journal of Plant Sciences
Year: 2004  |  Volume: 3  |  Issue: 1  |  Page No.: 31 - 35

In vitro Rapid Regeneration from Cotyledon Explant of Native-olive (Elaeocarpus robustus Roxb.)

M.M. Rahman, M.N. Amin and R. Ahmed    

Abstract: In the present study an efficient tissue culture method for high rate of shoot regeneration was developed for Elaeocarpus robustus. Cotyledonary explants with and without petiolar end were cultured in ½ MS medium supplemented with auxin and cytokinin. Explant with the petiolar/proximal end showed a better response than those without it in respect of shoot initiation. Half strength of MS medium supplemented with 1.0 mg l-1 BA+0.5 mg l-1 2,4-D was found to be best for producing organogenic callus. The best shoot proliferation was observed when cotyledon-derived callus was subcultured in medium fortified with a combination of 1.0 mg l-1 BA+0.5 mg l-1 Kn+0.1 mg l-1 NAA. In vitro elongated shoots were rooted with 80% success by treating them ½ in MS medium supplemented with 0.2 mg l-1 IBA and incubated under 30°C in dark for initial one week. Rooted shoots (plantlets) were successfully established into soil and the survival rate of the plantlets was about 55%.

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