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Asian Journal of Plant Sciences

Year: 2004  |  Volume: 3  |  Issue: 1  |  Page No.: 31 - 35

In vitro Rapid Regeneration from Cotyledon Explant of Native-olive (Elaeocarpus robustus Roxb.)

M.M. Rahman, M.N. Amin and R. Ahmed

Abstract

In the present study an efficient tissue culture method for high rate of shoot regeneration was developed for Elaeocarpus robustus. Cotyledonary explants with and without petiolar end were cultured in ½ MS medium supplemented with auxin and cytokinin. Explant with the petiolar/proximal end showed a better response than those without it in respect of shoot initiation. Half strength of MS medium supplemented with 1.0 mg l-1 BA+0.5 mg l-1 2,4-D was found to be best for producing organogenic callus. The best shoot proliferation was observed when cotyledon-derived callus was subcultured in medium fortified with a combination of 1.0 mg l-1 BA+0.5 mg l-1 Kn+0.1 mg l-1 NAA. In vitro elongated shoots were rooted with 80% success by treating them ½ in MS medium supplemented with 0.2 mg l-1 IBA and incubated under 30°C in dark for initial one week. Rooted shoots (plantlets) were successfully established into soil and the survival rate of the plantlets was about 55%.

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