In vitro Regeneration of Plantlets of Curcuma longa Linn. A Valuable Spice Plant in Bangladesh
Sprouted rhizome buds were collected from the field grown one year old rhizomes and surface sterilized by treating them with 0.1% HgCl2 for 14 min. The dissected rhizome bud explants (ca.1 cm) of turmeric were cultured in MS medium supplemented with different concentrations and combinations of cytokinin and auxin. The best result towards development of multiple shoot from the cultured explants was obtained when MS medium was supplemented with 2.0 mg l-1 of BA. Rooting experiments with half strength of MS medium and various concentrations of NAA, IBA and IAA revealed that 0.1-1.0 mg l-1 of any auxins was found to be effective for rooting. But root forming performance of IBA was proved to be the best among the three auxins tested. Rooted shoots (plantlets) were gradually acclimatized and successfully established in plastic pot containing garden soil under natural condition. About 70% of the transplanted plantlets survived and were eventually established in the field.
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