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Asian Journal of Plant Sciences
Year: 2004  |  Volume: 3  |  Issue: 1  |  Page No.: 25 - 30

Cloning and Sequencing of an ACC Synthase cDNA from Asparagus Spears and its Expression During Storage

Pankaj Kumar Bhowmik, Toshiyuki Matsui and A.K.M. Shameem Alam    

Abstract: A 1-aminocyclopropane-1-carboxylate (ACC) synthase cDNA from Asparagus spears was isolated and sequenced as a continuation after cloning, sequencing and expression analysis of ACC oxidase. The partial cDNA clone encoded an mRNA of 968 bp and the derived amino acid sequence was highly homologous to ACC synthase from bamboo banana, rice and wheat. Northern blot analysis showed increase level of pAS-ACS mRNA until 8 h at 20°C, which coincided with ethylene production and the ACC synthase activity, suggesting that the increase might be a response to the wounding associated with harvest.

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